DIY Bacterial Genome Engineering CRISPR Kit

Description

Are you curious as to what this CRISPR thing is about? Why it could revolutionize genetic engineering and change the world? Included in this kit is everything you need to engineer the genome of bacteria at home including Cas9, tracrRNA, crRNA and Template DNA template for an example experiment.

Included in this kit are a example experiments to introduce a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.

Kit contains enough materials for 5 experiments or more

LB Agar
LB Strep/Kan/Arabinose Agar
Glass bottle for pouring plates
Non-pathogenic E. coli bacteria
Inoculation Loops/Plate Spreader
10-100uL variable volume adjustable pipette(1uL increments)
Box of 96 Pipette Tips
14 Petri Plates
Microcentrifuge tube rack
Nitrile Gloves
Microcentrifuge tubes
50mL Tube for measuring
Bacterial transformation buffer 25mM CalCl2, 10% PEG 8000
LB Media for transformation recovery
Cas9 plasmid
gRNA plasmid
Template DNA
Protocol For Experiment